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cd11b antibody  (Miltenyi Biotec)


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    Miltenyi Biotec cd11b antibody
    Cd11b Antibody, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 98/100, based on 19 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+antibody/pmc13129426-4-0-10?v=Miltenyi+Biotec
    Average 98 stars, based on 19 article reviews
    cd11b antibody - by Bioz Stars, 2026-07
    98/100 stars

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    cd11b microbead  (Miltenyi Biotec)
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    Miltenyi Biotec cd11b microbead
    Representative workflow of isolation and culture of adult mouse microglia (A and B) Dissect the brain into small pieces on ice in Petri dish. (C) Collect cell pellets in C-tubes following mechanical/enzymatic dissociation using a gentleMACS dissociator. (D) Preparation of cell straining and debris removal processes. (E) Perform debris removal by carefully overlaying ice-cold PBS onto the cell suspension. (F) After centrifugation, identify three layers; the middle, yellowish layer corresponds to myelin dna debris. (G) Enrich <t>CD11b+</t> cells by magnetic separation using appropriate columns. (H) Seed isolated microglia onto 6-wll culture plates for downstream assays.
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    Representative workflow of isolation and culture of adult mouse microglia (A and B) Dissect the brain into small pieces on ice in Petri dish. (C) Collect cell pellets in C-tubes following mechanical/enzymatic dissociation using a gentleMACS dissociator. (D) Preparation of cell straining and debris removal processes. (E) Perform debris removal by carefully overlaying ice-cold PBS onto the cell suspension. (F) After centrifugation, identify three layers; the middle, yellowish layer corresponds to myelin dna debris. (G) Enrich CD11b+ cells by magnetic separation using appropriate columns. (H) Seed isolated microglia onto 6-wll culture plates for downstream assays.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Representative workflow of isolation and culture of adult mouse microglia (A and B) Dissect the brain into small pieces on ice in Petri dish. (C) Collect cell pellets in C-tubes following mechanical/enzymatic dissociation using a gentleMACS dissociator. (D) Preparation of cell straining and debris removal processes. (E) Perform debris removal by carefully overlaying ice-cold PBS onto the cell suspension. (F) After centrifugation, identify three layers; the middle, yellowish layer corresponds to myelin dna debris. (G) Enrich CD11b+ cells by magnetic separation using appropriate columns. (H) Seed isolated microglia onto 6-wll culture plates for downstream assays.

    Article Snippet: CD11b Microbead (Clone: M1/70.15.11.5) (1:10 ratio) , Miltenyi Biotech , Cat#130-049-601; RRID: AB_2927911.

    Techniques: Isolation, Suspension, Centrifugation

    Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Journal: STAR Protocols

    Article Title: Protocol for isolating and culturing microglia from the adult mouse brain using a magnetic-activated cell sorting system

    doi: 10.1016/j.xpro.2026.104471

    Figure Lengend Snippet: Flow cytometry for MACS-isolated microglia purity (A and B) Total CD11b-positive cells from MACS columns; (C) Cell viability assessed by Zombie Red staining; (D) Infiltrating leukocytes, including neutrophils (Ly6G+) and T lymphocytes (CD3+); (E) Proportions of microglia (CD11b+CD45low) versus monocytes/border-associated macrophages (CD11b+CD45high); (F) Proportion of resting (homeostatic) microglia identified as CD11b+TMEM119+ cells.

    Article Snippet: CD11b Microbead (Clone: M1/70.15.11.5) (1:10 ratio) , Miltenyi Biotech , Cat#130-049-601; RRID: AB_2927911.

    Techniques: Flow Cytometry, Isolation, Staining