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cd11b antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd11b antibody, anti-human, reafinity
    Cd11b Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd11b+antibody/CD11b+Antibody%2C+anti-human%2C+REAfinity/custom%40130-135-497%4042502392
    Average 93 stars, based on 6 article reviews
    cd11b antibody, anti-human, reafinity - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Distinct reduction in relative microglial glucose uptake compared to astrocytes and neurons upon isolation from the brain environment
    Article Snippet: .. Microglia were stained with 1.5 μL of CD11b Antibody, linked with VioBlue® (Miltenyi Biotec, 130–113-810) or APC (BioLegend®, 101,212). .. Astrocytes were stained with 1.5 μL of ACSA2 Antibody, linked with APC (Miltenyi Biotec, 130–116-142) or PE-Vio® 615 (Miltenyi Biotec, 130–116-249).

    other:

    Article Title: Protocol to sequentially isolate mouse oligodendrocytes, microglia, endothelial cells, astrocytes, and neurons via magnetic cell sorting
    Article Snippet: CD11b antibody, anti-mouse, REAfinity, Vioblue, clone REA592 | M1/70.15.11.5 – 1:50 dilution , Miltenyi , 130-113-810.

    Incubation:

    Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination
    Article Snippet: .. Resuspended cells were incubated with CD11b antibody-coated magnetic beads (130-097-142, Miltenyi Biotec, Germany) at 4°C for 15 min. CD11b + cells were resuspended with PBS and incubated with mouse F4/80 (F21480A02, Lianke Bio, China) and CD206 antibody (53-2061-80, Thermo Fisher Scientific, USA) for 30 min at 4°C in the dark, and detected by a flow cytometer. .. Non-denaturing lysis buffer (Solarbio, Beijing, China) was used to extract total protein, and the concentration of protein was determined by BCA kit.

    Article Title: Intestinal macrophages modulate synucleinopathy along the gut-brain axis.
    Article Snippet: Sebastiaan De Schepper1,19 ✉, Viktoras Konstantellos, James A. Conway, Dimitra Sokolova, L ud ov ic a Zaccagnini, Matthew V. Cowley, A nn er ie ke Sierksma, Maria Yudina, Marisa Edmonds, D ar ia Gavriouchkina, Bethany Geary, Amber Wallis, Meral Celikag, Zeynep Baykam, Mónica Vara-Pérez, Gerard C ro wl ey 1 , F ab ian Tobias Hager, Mitchell Bijnen, David Posner, Kelvin Luk, Vuk Cerovic, M en na Clatworthy, E li za be th J. Videlock, Z a n e Jaunmuktane, Kiavash Movahedi, Melanie Greter, Benny Chain, Dario R. Alessi, Soyon Hong1,21 ✉ & Tim Bartels1,21 ✉

    Article Title: Intestinal macrophages modulate synucleinopathy along the gut–brain axis
    Article Snippet: .. The resulting cell suspension was incubated with a CD11b antibody (Miltenyi Biotec) for 30 min at 4 °C to label CD11b + cells. ..

    Article Title: Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging
    Article Snippet: After enzymatic digestion, 0.5 ml of heat-inactivated FBS (to 10% of final concentration) was added to neutralize trypsin, and tissue was mechanically triturated using a 1 ml pipette, filtered through a 40 μm cell strainer (Corning 352340), pelleted at 400xg for 5 minutes and washed twice with HBSS. .. Dissociated cells were resuspended in MACS buffer (HBSS with 0.5% BSA) with addition of 0.5 mg/ml DNAse and incubated with CD11b antibody (Miltenyi Biotec, 130-097-142) for 15 minutes at 40C. .. After the incubation, cells were washed with 10 ml of HBSS, pelleted at 400xg for 5 minutes and loaded on LS columns (Miltenyi Biotec, 130-042-401) in 0.5 ml of MACS buffer on the magnetic stand.

    Magnetic Beads:

    Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination
    Article Snippet: .. Resuspended cells were incubated with CD11b antibody-coated magnetic beads (130-097-142, Miltenyi Biotec, Germany) at 4°C for 15 min. CD11b + cells were resuspended with PBS and incubated with mouse F4/80 (F21480A02, Lianke Bio, China) and CD206 antibody (53-2061-80, Thermo Fisher Scientific, USA) for 30 min at 4°C in the dark, and detected by a flow cytometer. .. Non-denaturing lysis buffer (Solarbio, Beijing, China) was used to extract total protein, and the concentration of protein was determined by BCA kit.

    Flow Cytometry:

    Article Title: The role of SH3RF2 in lung squamous cell carcinoma and M2 polarization: insights into LZTS2 ubiquitination
    Article Snippet: .. Resuspended cells were incubated with CD11b antibody-coated magnetic beads (130-097-142, Miltenyi Biotec, Germany) at 4°C for 15 min. CD11b + cells were resuspended with PBS and incubated with mouse F4/80 (F21480A02, Lianke Bio, China) and CD206 antibody (53-2061-80, Thermo Fisher Scientific, USA) for 30 min at 4°C in the dark, and detected by a flow cytometer. .. Non-denaturing lysis buffer (Solarbio, Beijing, China) was used to extract total protein, and the concentration of protein was determined by BCA kit.

    Suspension:

    Article Title: Intestinal macrophages modulate synucleinopathy along the gut-brain axis.
    Article Snippet: Sebastiaan De Schepper1,19 ✉, Viktoras Konstantellos, James A. Conway, Dimitra Sokolova, L ud ov ic a Zaccagnini, Matthew V. Cowley, A nn er ie ke Sierksma, Maria Yudina, Marisa Edmonds, D ar ia Gavriouchkina, Bethany Geary, Amber Wallis, Meral Celikag, Zeynep Baykam, Mónica Vara-Pérez, Gerard C ro wl ey 1 , F ab ian Tobias Hager, Mitchell Bijnen, David Posner, Kelvin Luk, Vuk Cerovic, M en na Clatworthy, E li za be th J. Videlock, Z a n e Jaunmuktane, Kiavash Movahedi, Melanie Greter, Benny Chain, Dario R. Alessi, Soyon Hong1,21 ✉ & Tim Bartels1,21 ✉

    Article Title: Intestinal macrophages modulate synucleinopathy along the gut–brain axis
    Article Snippet: .. The resulting cell suspension was incubated with a CD11b antibody (Miltenyi Biotec) for 30 min at 4 °C to label CD11b + cells. ..

    Magnetic Cell Separation:

    Article Title: Heterochronic myeloid cell replacement reveals the local brain environment as key driver of microglia aging
    Article Snippet: After enzymatic digestion, 0.5 ml of heat-inactivated FBS (to 10% of final concentration) was added to neutralize trypsin, and tissue was mechanically triturated using a 1 ml pipette, filtered through a 40 μm cell strainer (Corning 352340), pelleted at 400xg for 5 minutes and washed twice with HBSS. .. Dissociated cells were resuspended in MACS buffer (HBSS with 0.5% BSA) with addition of 0.5 mg/ml DNAse and incubated with CD11b antibody (Miltenyi Biotec, 130-097-142) for 15 minutes at 40C. .. After the incubation, cells were washed with 10 ml of HBSS, pelleted at 400xg for 5 minutes and loaded on LS columns (Miltenyi Biotec, 130-042-401) in 0.5 ml of MACS buffer on the magnetic stand.



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